What Happens When You Actually Test an Anti-Aging Serum? We Did - Here's the data

At 28N, we believe in transparency, efficacy, and letting the results speak for themselves. Skincare, after all, is part ritual and part science—and we’re committed to delivering on both fronts.

We recently put our A⁶ Anti-Ageing Formula through its paces in a controlled, independent in-vivo study. The goal? To measure the visible changes in skin texture, radiance, and overall vitality over 28 days. The test was conducted under lab-controlled conditions (think: stable temperature and humidity, precision imaging tools, and no makeup allowed). Here’s what went down.

The Setup: A Real-World-ish Lab Trial

Fourteen women, ages 20 to 55, used our A⁶ Anti-Ageing Formula twice a day—once in the morning, once at night—after cleansing. Half of them used it solo, and the other half paired it with our Vitamin C Booster, the way some of our customers already do IRL. 

Measurements were taken on days 0, 7, 14, and 28 using devices like the VisioFace 1000D (for wrinkles), the Skin-Glossymeter GL 200 (for radiance), and the Mexameter MX 18 (for redness). Yes, those are real devices, and yes, they sound kind of sci-fi—but we’re here for it.

So… Does It Work?

Spoiler: Yes. The numbers are good-good. Let’s break them down.

If You’re a Minimalist: Anti-Aging Serum Alone

Even without layering, the serum delivered visible results.

• Wrinkle Volume: Down 39.08% by Day 28
• Wrinkle area ratio: Down 24.13%
• Wrinkle Depth: Down 23.43%
• Skin Gloss (aka radiance): Up 18.23%

Basically, your fine lines look finer, your skin calms down, and you get that low-key glow people pay facials for.

If You’re a Maximalist: with Vitamin C Booster

The duo performed even better when paired.

• Wrinkle Volume: Down 47.47% by Day 28
• Wrinkle area ratio: Down 47.15%
• Wrinkle Depth: Down 44.76%

So yes, the Vitamin C Booster takes things up a notch—especially on soothing redness. If you’re prone to sensitivity or dullness, the combo is your power move.

Our Favorite Part? The Visuals.

Photos don’t lie. The study captured 3D images of crow’s feet and skin redness over time, and the results are the kind of subtle-but-wow transformation we all want: smoother, brighter, calmer skin that still looks like you.

Image 1: Redness Reduction ( A⁶ Anti-Ageing Formula with Vitamin C Booster)

This visual tracks the change in facial redness over a 28-day period for two subjects. Each row shows one subject’s progress across four time points: Day 0 (before use), Day 7, Day 14, and Day 28. Red areas indicate high levels of skin redness or inflammation, while blue and purple tones represent calmer, less inflamed skin.

• Subject 1: Starts with noticeable red clusters on Day 0. By Day 28, redness has significantly diminished, replaced mostly with cooler blue tones.
• Subject 2: Begins with a dense spread of redness, especially central in the image. There’s a marked decrease over time, with Day 28 showing far more purple-blue skin and only mild residual red.

Bottom line: These visuals show a clear reduction in erythema (redness), confirming the serum’s soothing and anti-inflammatory effects—especially when paired with the Essence Toner.

Image 2: 3D Eye Wrinkle Depth ( A⁶ Anti-Ageing Formula with Vitamin C Booster

This set shows 3D surface scans of the eye area to assess wrinkle depth and volume over time. Green/yellow/red gradients indicate the severity and depth of wrinkles, with red being deepest.

• Subject 1: The red “troughs” of deep-set wrinkles in the Day 0 scan gradually recede. By Day 28, the contours are flatter, with more green (shallow) areas and less red.
• Subject 2: Similarly, deep eye lines are visibly prominent at the start. Over 28 days, the scans show smoother surfaces with reduced yellow and red zones, indicating a shallower wrinkle profile.

Bottom line: The scans provide clear visual evidence that the serum, especially when used with the toner, visibly reduces wrinkle depth and improves skin texture over time.

Why A⁶?

The "A" stands for Anti-Ageing, while the "6" represents the six scientifically selected active ingredients at the heart of the formula.

Rather than relying on a single anti-ageing ingredient, A⁶ was designed to target multiple visible signs of skin ageing through complementary mechanisms—helping improve the appearance of wrinkles while supporting skin hydration, firmness and overall skin quality.

• Cycloretin™ – A next-generation retinoid alternative designed to support smoother, younger-looking skin with excellent tolerability.
• Bakuchiol – Helps support skin renewal and improve the appearance of fine lines and wrinkles.
• Amplified Argireline™ – A peptide that helps reduce the appearance of expression lines.
• Collagen Amino Acids – Help support skin hydration and maintain a healthy-looking skin surface.
• Peptide Complex – Supports firmer, smoother-looking skin and helps improve visible skin texture.
• Ultra-Low Molecular Weight Hyaluronic Acid – Helps deliver deep hydration for a plumper, healthier-looking complexion.

 

The Takeaway: Visible Anti-Ageing, Backed by Independent Testing

It's one thing to hear that a product helps reduce the appearance of wrinkles. It's another to see measurable improvements in wrinkle volume, wrinkle depth and skin radiance demonstrated through an independent 28-day in-vivo study.

A⁶ Anti-Ageing Formula was designed to target multiple visible signs of skin ageing rather than relying on a single active ingredient. By combining six scientifically selected ingredients, it helps improve the appearance of fine lines while supporting smoother, healthier-looking skin.

Whether used on its own or paired with our Vitamin C Booster, the results show that a multi-pathway approach can deliver meaningful visible improvements in just 28 days.

Ready to experience it for yourself?

Shop the A⁶ Anti-Ageing Formula

Looking for the Complete Routine?

Our study also showed that pairing A⁶ Anti-Ageing Formula with our Vitamin C Booster delivered even greater improvements in the appearance of wrinkle volume and wrinkle depth than using A⁶ alone.

If you're looking for a complete anti-ageing routine, we've already put it together for you.

The Anti-Ageing Routine combines:

• Glycolipids Milk Wash – A gentle, barrier-first cleanser that prepares the skin without stripping its natural lipids.
• Vitamin C Booster – Antioxidant support to help defend against oxidative stress while enhancing skin radiance.
• A⁶ Anti-Ageing Formula – A six-active formula designed to target multiple visible signs of skin ageing.

Together, these three steps create a complete routine designed to support smoother, brighter, healthier-looking skin while maintaining barrier integrity.

Ready to upgrade your routine?

Shop the Anti-Ageing Routine →